semaglutide recombinant synthesis method of - Eureka High-yield recombinant production of the

Feed Preparation: Feed medium of 12 liters for fermentation was prepared according to the components of Table 3 and sterilized

Lane 1: product of semaglutide main chain P29 with SNAC and DDDDK tags after Ni2+-assisted cleavage (Cleavage 1)

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The synthesis method of the acylated GLP-1 compound and modified gene thereof is characterized by comprising the following steps of preparing a compound 2, namely, taking GLP-1 (9-37) as an initial raw material, carrying out PEG medication by a compound 1 on Lys of a position 26 of a relative sequence GLP-1 (9-37), controlling the reaction pH and carrying out an acylation coupling reaction to obtain a compound 2